| The Effect of Extract of Paeonia lactiflora on the Improvement of Ischemic Acute Renal Failure |
| Hyun Joo Jung, M.S.1, Min-Ji Kim, M.S.1, Sun Ha Lim, M.S.3, Jun Hong Park, M.S.4, Hyeong-Kyu Lee, Ph.D.5, Ki Sung Ahn, M.D., Ph.D.2 and Jongwon Lee, Ph.D.1 |
Departments of Biochemistry1
Internal Medicine2
School of Medicine Catholic University of Daegu, Daegu; Hypoxi Co., Ltd., Daegu3 Gyeongbuk Agricultural Technology Administration4
Gyeongbuk Natural Medicines Research Center5
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea |
| 임상연구 : 허혈성 급성신부전에 대한 작약추출물의 영향 |
| 정현주1, 김민지1, 임선하3, 박준홍4, 이형규5, 안기성2, 이종원1 |
| 대구가톨릭대학교 의과대학 생화학교실1, 내과학교실2, (주)하이폭시3, 경상북도농업기술원4, 한국생명공학연구원 바이오신약 연구부5 |
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| Abstract |
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Purpose:The present study was undertaken to investigate whether the extract or the ethyl acetate fraction of Paeonia lactiflora, which improves cell survival under ischemic condition by inhibiting apoptosis, can prevent ischemic acute renal failure, using rats as an animal model.
Methods:In the control group, ischemia/reperfusion (I/R) injury was induced in male Sprague-Dawley rats by clamping of left renal pedicle for 45 minutes after removal of the right kidney, and subsequent reperfusion of the pedicle for 24 hours. In the experimental group, the water extract or ethyl acetate fraction of methanol extract Paeonia lactiflora was injected 1 hour prior to ischemia/reperfusion injury. We measured serum concentrations of creatinine at 24 hours after I/R injury. And the kidneys were extracted, fixed in a 10 % neutral-buffered formalin solution, embedded in paraffin and used for histopathological examination.
Results:Rats in the experimental group, treated with water extract or ethyl acetate fraction of methanol extract of Paeonia lactiflora, exhibited significant decrease in the serum concentrations of creatinine (approx. 2.0 mg/dL), compared with those in the control group (approx. 4.0 mg/dL). Finally, the cell morphology of the kidney of rats treated with ethyl acetate fraction of Paeonia lactiflora was well preserved, when judged from histopathological point of view.
Conclusion:Pretreatment of rats with the water extract or ethyl acetate fraction of Paeonia lactiflora might be beneficial for the treatment of acute renal failure in humans. |
| Key Words:
Renal failure, Peony, Apoptosis |
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