| Association of Transforming Growth Factor-β1 and Vascular Endothelial Growth Factor Gene Polymorphisms with Chronic Allograft Nephropathy and Graft Survival in Korean Renal Transplant Recipients |
| Ji-Hyung Cho, M.D., Hye-Myung Ryu, Ph.D., Mi-Kyung Jin, M.D., Joo-Hyun Chun, M.D., Seung-Hyea Hyun, M.D., Ji-Young Choi, M.D., In-Kyong Hur, M.D., Eun-Young Lee, M.D., Sun-Hee Park, M.D., Yong-Lim Kim, M.D. and Chan-Duck Kim, M.D. |
| Department of Internal Medicine, Kyungpook National University Graduate School of Medicine, Daegu, Korea |
| 임상연구 : 한국인 신이식 수혜자에서 transforming growth factor-β1 및 vascular endothelial growth factor 유전자의 단일염기다형성이 만성 이식신 신병증과 이식신 생존율에 미치는 영향 |
| 조지형, 류혜명, 진미경, 전주현, 현승혜, 최지영, 허인경, 이은영, 박선희, 김용림, 김찬덕 |
| 경북대학교 의학전문대학원 내과학교실 |
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| Abstract |
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Purpose : Transforming growth factor-β1 (TGF-β1) has been associated with the promotion of renal allograft interstitial fibrosis and thereby chronic allograft nephropathy (CAN). Vascular endothelial growth factor (VEGF) has been shown to contribute to cytoprotection of the graft after kidney transplantation. We investigated the influence of single nucleotide polymorphisms (SNPs) of the TGF-β1(C-509T and T869C) and the VEGF gene (C-2578A and C405G) on graft survival and the development of CAN.
Methods : Genotyping was carried out using a real-time polymerase chain reaction which was performed on the LightCycler480 in 221 Korean renal transplant recipients and 148 healthy controls. According to the presence of CAN or chronic calcineurin inhibitor nephrotoxicity, the recipients were separated into the CAN (n=21) and the No CAN (n=200) groups.
Results : The genotype frequencies of the SNPs were in Hardy-Weinberg equilibrium. The distributions of genotypes and alleles did not differ between recipients and controls. No significant differences were observed in the genotype distributions and allele frequencies between the CAN and the No CAN groups. The frequencies of haplotypes were not significantly different between the two groups, either. There were no statistically significant effects of TGF-β1 and VEGF gene polymorphisms on graft survival.
Conclusion : This study did not show any statistically significant effects of four selected SNPs of the TGF-β1 and the VEGF genes on the development of CAN and graft survival in Korean renal transplant recipients.
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| Key Words:
Transforming growth factor-β1, Vascular endothelial growth factor, Single nucleotide |
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