Korean Journal of Nephrology 2000;19(2):218-226.

원저 : Puromycin aminonucleoside로 유발된 흰쥐에서의 사구체 비후 및 경화증의 형태학적 변화

고철우(Cheol Woo Ko),구자훈(Ja Hoon Koo),김승미(Sung Mi Kim),장희숙(Hee Suk Jang),홍인희(In Hee Hong),김용진(Young Jin Kim)

Abstract

Purpose
: This experimental study was conducted to determine serial morphological changes of rat's kidney with chronic puromycin aminonucleoside (PAN) nephropathy. Special emphasis was given to the occurrence of glomerular hypertrophy and its relationship to the subsequent development of focal segmental glomerulosclerosis(FSGS). Methods: Sprague-Dawley rats weighing 200- 230g were used and divided into control(n=9) and experimental group(n=15). Rats were given subcuta-neous injections of PAN at a dose of of 2mg/100g body weight, or an equivalent volume of normal saline and six injection were given over a period of 9 weeks, at weeks 0, 1, 3, 5, 7 and 9. At weeks 4, 8 and 11, rats were sacrificed and kidney weight, kidney weight/body weight(%) and various laboratory tests including serum protein and albumin were determined. Renal tissues were prepared with Histo-choice fixative and paraffin embedding for morpho-logic study. Results : Kidney weight and kidney weight/body weight(%) were increased significantly in experimental group compared to controls at 4, 8 and 11 weeks. Heavy proteinuria along with lowering of serum protein and albumin and elevation of serum cholesterol was seen in experimental group at week 4 and this change became more marked on weeks 8 and 11. The frequency of FSGS in experimental animal, at week 4, 8 and 11 were 0.6%, 10.6% and 26.2% respectively(p<0.05) and the development of FSGS was more marked in juxtamedullary glomeruli compared to cortical glomeruli. Glomerular surface area showed significant increase in experimental animals compared to controls(p<0.01), the percentage of increase being 12.0, 14.7 and 12.3% at week 4, 8 and 11. And the surface areas of juxtamedullary glo-meruli were larger than those of cortica1 glomeruli throughout the study period. Conclusion: In summary, present study indicates that glomerular hypertrophy occurs and precedes the development of FSGS in rats with chronic PAN nephropathy and juxtamedullary glomeruli are more susceptible to developing FSGS compared to cortical glomeuli.