Korean Journal of Nephrology 2011;30(5):443-451.
The Role of Adipose Tissue-Derived Stem Cells in Ischemic Acute Renal Injury in Rats
Woo Jung Sung, M.D.1, Yong-Jin Kim, M.D.2, Yong Hoon Park, M.D.3 and Keuk-Jun Kim, Ph.D.4
Department of Pathology1
Catholic University of Daegu, School of Medicine, Daegu, Korea Department of Pathology2 and Pediatrics3
Yeungnam University, College of Medicine, Daegu, Korea ; Department of Clinical Pathology4
Tae Kyeung College, Gyeongsan, Korea
원저 : 허혈성 급성 신부전증 실험쥐에 투여한 지방유래 줄기세포의 역할
성우정1, 김용진2, 박용훈3, 김극준4
대구가톨릭대학교 의과대학 병리학교실1 , 영남대학교 의과대학 병리학교실2, 영남대학교 의과대학 소아청소년과학교실3 , 대경대학교 임상병리과4
Abstract
Purpose: The aim of this study is to explore the role of human adipose tissue-derived adult stem cells (ASCs) in repair of acute ischemic renal injury. Methods: Acute ischemic tubular necrosis was induced in Spraque-Dawley rats (n=15) by clamping bilateral renal pedicles for 40 minutes. Before releasing the clamps, iron labeled stem cells (1×106/0.3 mL) were directly injected to one side of renal cortex and phosphate-buffered saline (PBS) injections were done as a control to the opposite kidney. The examination on both kidney was done after 1, 2 and 4 weeks (n=5 each). The evaluation of the degree of tubular necrosis and the location of stem cells was done by light and electron microscopically. Results: The rats were all alive without rejection. Morphologically, recovery rate was increased up to about 20% in ASCs injection group in 2 weeks-group (p<0.001). Few Prussian-blue positive cells were demonstrated in 2 weeks-group and more cells were present in 4 weeks-group. The cells which were positively stained in Prussian-blue stain were mainly located in peritubular spaces. Electron microscopically, fine iron granules with electron density were found in the cytoplasm of peritubular endothelial cells. Conclusion: As a result, the endothelial cells might be differentiated from injected ASCs. It seems that stem cells help repairing the tubular epithelium in acute renal failure model, not by tubulogenesis but by vasculogenesis. This outcome suggests that the possibility of therapeutic use of ASCs without immunosuppression of recipients in acute ischemic renal injury.
Key Words: Mesenchymal stem cells, Acute renal failure


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