| Erythropoietin (EPO) Attenuates Renal Injury in an Experimental Model of Cisplatin-induced Nephrotoxicity in Rats |
| Dae Eun Choi, M.D.1, Sarah Chung, M.D.1, Young Mo Lee, M.D., Ph.D.2, Kwang-Sun Suh, M.D., Ph.D.3, Ki-Ryang Na, M.D., Ph.D.1, Young-Tai Shin, M.D., Ph.D.1 and Kang Wook Lee, M.D., Ph.D.1 |
Department of Internal Medicine1
Chungnam National University Hospital, Chungnam Department of Internal Medicine2
Korea University Guro Hospital, Seoul Department of Pathology3
Chungnam National University Hospital, Chungnam, Korea |
| 기초연구 : Erythropoietin (EPO) Attenuates Renal Injury in an Experimental Model of Cisplatin-induced Nephrotoxicity in Rats |
| Dae Eun Choi, M.D.1, Sarah Chung, M.D.1, Young Mo Lee, M.D., Ph.D.2, Kwang-Sun Suh, M.D., Ph.D.3, Ki-Ryang Na, M.D., Ph.D.1, Young-Tai Shin, M.D., Ph.D.1 and Kang Wook Lee, M.D., Ph.D.1 |
| Department of Internal Medicine1, Chungnam National University Hospital, Chungnam Department of Internal Medicine2, Korea University Guro Hospital, Seoul Department of Pathology3, Chungnam National University Hospital, Chungnam, Korea |
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| Abstract |
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Purpose:In addition to its hematopoietic effects, EPO has protective effects in vivo in several animal models of acute renal injury. We examined whether EPO also attenuated renal injury in a rat model of cisplatin-induced nephrotoxicity via anti-apoptotic and anti-inflammatory actions.
Methods:Male SpragueDawley rats were divided into four groups: control rats, EPO+control rats, cisplatin rats, and EPO+cisplatin rats. EPO treatment was started 24 h prior to cisplatin administration. Then, 96 h after cisplatin administration, all experimental animals were killed. And renal molecular, functional and structural parameters were measured.
Results:The serum levels of BUN and creatinine in the 96 h after cisplatin administration were significantly lower than in cisplatin rats. On microscopic examination, the magnitude of renal tubular epithelial damage in the EPO+cisplatin rats was also significantly less than that of cisplatin rats. Renal expression of TNF-α, Fas, MCP-1 and TGF-β in the cisplatin rats was significantly higher than those of control rats and EPO+control rats. The levels of TNF-α, Fas, MCP-1 and TGF-β gene expression in EPO+cisplatin rats were significantly lower than those of cisplatin rats. The Bcl-2 mRNA level in EPO+cisplatin rats was significantly higher than in cisplatin rats. EPO+cisplatin rats had significantly fewer TUNEL-positive cells.
Conclusion:These results suggest that EPO has a protective effect against experimental cisplatin- induced renal injury and that the anti-inflammatory and anti-apoptotic properties of EPO may be involved. |
| Key Words:
Apoptosis, Cisplatin, Erythropoietin, Inflammation |
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