Korean Journal of Nephrology 1998;17(5):661-666.

Dexamethasone, Transforming Growth Factor-β1이 사구체 내피세포의 Vascular Cell Adhesion Molecule-1(VCAM-1) 발현에 미치는 영향 (The Effect of Dexamethasone and Transforming Growth Factor-β1 on the Cytokine Induced Expression of VCAM-1 in Glomerular Endothelial Cells)

정영옥, 장윤혜, 양원석, 장혜숙, 안한종, 이재담, 박수길, Su Kil Park (Young Ok Jung, Jong Soo Lee, Yoon Hye Chang, Won Seok Yang, Hye Sook Chang, Hanjong Ahn and Jae Dam Lee)

Abstract

Endothelial cell have been shown to play an active role in many phases of immunologic process, including binding of T lymphocytes, neutrophils, platelets, and monocytes to the endothelium, as well as phagocytosis. Endothelial cells can also serve as targets that undergo cell injury. The most prominent mediators of endothelial cell activation are IL-1β and TNF-α. VLA-4 was first identified as an endothelial cell surface receptor. We performed the culture of endothelial cells derived from human glomerular capillaries and studied the cytokine-regulated expression of VCAM- 1 , and the effect of dexamethasone and TGF-β on the cytokine induced VCAM-1 expression using ELISA method. Expression of VCAM-1 was not detectable above background level in the basal unstimulated state. However, VCAM-1 was rapidly induced after exposure to IL-1β(5ng/ml) or TNF-α(1, 10ng/ml) (O.D.=1.76±0.15, 1.95±0.35, 1.88±0.17, mean±S.E., control=0.36±0.028, n=8-24, P<0.05). But IFN-γdid not increase the expression of VCAM-1. Addition of dexamethasone(10μM) and TGF-β1(1ng, 10ng/ml) blunted IL-1β and TNF-α induced expression of VCAM-1. Therefore, VCAM-1 could be inducible in human glomerular endothelial cells, and it was regulated by dexamethasone and TGF-β1. The negative findings in histopathology may reflect the transience of VCAM-1 expression and does not necessarily exclude an important role of this molecule in the early stages of renal disease.