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| 원저 : 흰쥐에서 카페인이 신장 Atrial Natriuretic Peptide 및 산화질소계에 미치는 영향 (Original articles : Enhanced Atrial Natriuretic Peptide and Nitric Oxide System Following the Treatment with Caffeine in Rats) |
| 하정훈(Jeong Hoon Ha),염충호(Chung Ho Yeum),김수완(Soo Wan Kim),김남호(Nam Ho Kim),최기철(Ki Chul Choi),이종은(Jong Un Lee) |
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| Abstract |
Purpose
: The present study was aimed to examine whether caffeine alters the local regulation of atrial natriuretic peptide(ANP) and nitric oxide(NO) systems in the kidney. Methods : Male Sprague-Dawley rats were treated with caffeine, consisting of a single oral bolus(0.2%, 1 mL/kg) followed by supplementation in drinking water(0.2%) for 1 day. Rats treated the same without caffeine served as control. The tissue expression of ANP mRNA was determined by reverse transcription-polymerase chain reaction. Tissue levels of nitrite/nitrate were determined. The expression of aquaporin(AQP)-1 and AQP2 proteins was determined by Western blot analysis. Results : Following the treatment with caffeine, the expression of ANP mRNA was increased in the kidney. The renal tissue nitrite/nitrate level was also increased by caffeine-treatment. On the other hand, the expression of AQP1 and AQP2 proteins was not significantly altered. Conclusion : The enhanced activities of local ANP and NO systems may in part be causally related with the caffeine-induced natriuresis and diuresis, while AQP channels are not involved. (Korean J Nephrol 2002;21(1):123-128) |
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