Korean Journal of Nephrology 2000;19(3):401-409.

원저 : 배양된 복막중피세포의 Fibronectin 생성에 있어 헤파린의 효과 (Effect of Heparin on the Production of Fibronection in Cultured Human Peritoneal Mesothelial Cells)

양원석(Won Seok Yang),박정식(Jung Sik Park),이상구(Sang Koo Lee),김순배(Soon Bae Kim),김병식(Byung Sik Kim)

Abstract

Interleukin-1β(IL-1β), which is released into the peritoneal cavity during peritonitis in continuous ambulatory peritoneal dialysis(CAPD) patients, stimulates the production of extracellular matrix proteins, including fibronectin and type, I collagen, in the cultured peritoneal mesothelial cells(HPMCs). This may participate in the development of peritoneal fibrosis developing after episodes of peritonitis. Heparin sti-mulates the fibrinolysis and it is a routine practice to put 0.5-1U/mL of heparin into the dialysis fluid to prevent the formation of fibrin clot. Besides the anticoagulant acivity, heparin has diverse biologic effects on the cells. In this study, we investigated the effect of heparin on the production of fibronectin, one of the extracellular matrix proteins, in the cul-tured human peritoneal mesothelial cells. Incubation of the cultured HPMCs with IL-1β(1 ng/mL) caused an increased expression of fibronectin mRNA by the cells. This stimulatory effect of IL-18 on the expression of fibronectin mRNA was inhibited by heparin, dose-dependently as well as time-depen-dently. The expression of fibronectin mRNA in the unstimulated cells was also inhibited by heparin. The amount of secreted fibronectin in the culture super- natant was decreased by heparin(1U/mL). This inhibitory effect was present regardless of the addition of IL-lβ. However, IL-lβ-induced increase in the expression of TGFβ1 mRNA in the cultured HPMCs was not inhibited by heparin. In conclusion, heparin inhibited the production of fibronectin by the cultured HPMCs at the concentration used clinically during peritonitis in CAPD patients. This suggests that heparin may have a preventive effect on the development of peritonitis associated peritoneal fibrosis as well as decrease the formation of fibrin clot.