Korean Journal of Nephrology 2000;19(6):1033-1040.

Puromycin Aminonucleoside로 유발된 신증후군성 단백뇨 백서에서 알부민 투여에 의한 신손상 (Renal Injury by Albmnin Infusion in Puromycin Aminonucleoside Induced Nephrotic-Range Proteinuric Rats)

성순희(Soon Hee Sung),이승주(Seung Joo Lee)

Abstract

Purpose
To examine the renal injury by albumin infusion, which has been widely used to correct severe nephrotic edema, in puromycin aminonucleoside(PAN) induced nephrotic-range proteinuric rats. Materials and Methods: Sixty Sprague-Dawley rats were divided into 3 experimental groups, control group(PAN only, n=20), low-dose albumin group (PAN+25% albumin 1g/kg, n=20) and high-dose albumin group(PAN+25% albumin 4g/kg, n=20). PAN was peritoneally injected on day #1 and #7 to all experimental rats & 25% albumin was peritoneally injected from day #3 for three weeks. Twenty-four hour urine protein, serum creatinine and serum albumin were measured weekly. Histopathologic and ultrastructural examination using transmission EM, and immunohistochemical stainings of CD68, pan T, and L26 were done at 3 and 8 week. Results: Twenty-four hour urine proteins at 2 and 3 weeks were significantly increased in high-dose albumin group compared to control and low-dose albumin group. Serum creatinine at 3 week in high-dose albumin group was 0.9±0.54mg/dL which was significantly higher than 0.6±0.3lmg/dL of control group and 0.6±O.43mg/dL of low-dose albumin group. Serum creatinine at 8 week in high-dose albumin group was 1.2±0.85mg/dL which was significantly higher than 0.6±0.24mg/dL of control group and 0.6±0.21mg/dL of low-dose albumin group. Serum albumin was not different among groups. Percent(%) glomerular sclerosis at 8 week in high-dose albumin group was 9.4±5.8% which was significantly higher than 3.5±1.7% of control group. Interstitial mononuclear cell infiltration was increased according to albumin dose and most of the was CD68(+) macrophages. Vacuolar degeneration of podocytes, accumulation of protein reabsorption droplets in the cytoplasm of podocytes and proximal tubule and lipid droplets in the cytoplasm of proximal tubules were increasd according to alburnin dose. Conclusion: Albumin infusion in PAN induced proteinuric rats load accelerate the decline in renal function by progressive glomerular and tubulo-interstitial injury. Albumin infusion in clinical nephrotic edema shoud be used very carefully.