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| 혈액투석에 의한 혈소판의 활성도 지표로서의 P - selectin 측정 |
| 김형직 , 구자룡 , 채동완 , 노정우 |
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| Abstract |
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Recent studies suggested that platelet activation with surface expression of P-selectin may be related to a loss of viability and early clearing from circulation by monocytes and macrophages of the reticuloendothelial system. We have studied platelet activation during hernodialysis with a cuprophane membrane in eighteen patients with uremia. Blood samples were obtained at the beginning of the hemo- dialysis(sample I ) and after 1 hr of hemodialysis (sample II), and at the end of hemodialysis from the venous line (sample III). Platelet surface expression of P-selectin(CD62) was determined by flow cyto- metry. Percentage of positive platelets(% PP) of P- selectin was measured 43.9±15.6 % in sample I, 36.9±16.0% in sample II, and 40.1±13.1% in sample Iil. No statistical differences in P-selectin expression were observed in sample I, II, and III throughout the period of hemodialysis although platelet P-selec- tin expression after 1 hour of hemodialysis showed a slightly decrease(P=0.44). However, a significant in- crease in fluorescence occurred in samples from ure- mic patients(43.0±15.6%) with respect to low fluore- scence was seen in normal control sera(14.61±1.2%). Our study shows that the substantial and irre- versible platelet P-selectin expression might be con- tributing factors in early clearing of platelet from circulation in uremic patients. |
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