Korean Journal of Nephrology 1998;17(3):383-390.

Erythropoietin이 사구체 내피세포아 제대정맥 내피세포의 endothelin 생성에 미치는 영향

박수길 , 김승후 , 양원석 , 김청수 , 안한종 , 박정식 , 이재담

Abstract

The administration of erythropoietin for renal failure patients is frequently associated with a mild-to-marked rise in arterial blood pressure. The erythropoietin-induced hypertension has been varia- bly attributed to the rise in erythrocyte concentration and/or a direct or indirect pressor action of erythropoietin on vascular smooth muscle. Especially erythropoietin-induced hypertension may be mediated by increased production of the potent vasoactive peptide endothelin. This study was designed to determine the effect of erythropoietin to the produc- tion of endothelin in human glomerular endothelial cells and human umbilical vein endothelial cells. ELISA method was used to measure the endothelin- 1, after 4 x 10' endothelial cells were grown to confluency on 24-well plates, in which erythropoietin 25u/ml was incubated for 24 hours. The results showed that the endothelin production in human glomerular endothelial cells was insignificant (116.0± 14.0%, P>0.06, mean S.E., n=5, each n means the mean of duplicate experiments), but the endothelin production in human umbilical endothelial cells was increased after 25u/ml erythropoietin treatment(126.5±15.2%, P<0.05, mean±S.E., n=4). In glomerular en- dothelial cells, TGF- B (0.5mg/ml) increased the pro- duction of endothelin(218,8±57.2%, P<0.01, mean± S.E., n=5), also it looks like that TNF- A and thrombin might increase the production of endothelin according to the concentration. In conclusion, the responsiveness of endothelial cells to erythropoietin may be different according to the cell type, and glomerular endothelial cells could increase the pro- duction of endothelin, if appropriate stimuli were given.