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| 쥐의 anti - Thy - 1 신염에서 Platelet - derived Growth Factor B와 Transrorming Growth Factor -B1 mPNA의 동시 발현 |
| 하일수 , 박혜원 , 안규리 , 정해일 , 최용 |
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| Abstract |
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In anti-Thy-1 nephritis induced in rats, augmen- ted expressions of platelet-derived growth factor B (PDGF B) and . Transforming growth factor-51 (TGF-01) have been reported, and the critical roles of these factors in the pathogenesis of the disease have been made clear. Yet, because the two growth factors have been analyzed separately in two experi- ments which had some dissimilarities in methods inducing nephritis as well as in periods of obser- vations, the exact order of the expressions of the factors are not clear. This study was performed to elucidate the chronologieal sequence of Mrna ex- pression of the two growth factors in one nephritis model. Anti Thy-1 nephritis was induced in Sprague- Dawley rats by intravenous injection of anti- tbymocyte serum (ATS) which had been produced in a rabbit immunized by rat thymocytes. Renal cortical tissues obtained from nephritic rats 1, 4, 7, and 14 days after ATS injection as well as control rats were subjected to the following studies; immu- nofluorescent studies for rabbit IgG, light microscopic observations for changes in glomerular cellularities and extent of mesangial matrix, measurement of quantitative changes in glomerular Mrna for PDGF B and TGF-B1 by Northern blot analyses. Induction of nephritis was confirmed by mesangial deposition of rabbit IgG on day 1, followed by the increase in cellularity and mesangial matrix on day 14. For PDGF B, 3 transcripts of different sizes(3.5-, 2.6- and 1.6-kb) appeared, and the 2.6-kb transcript was the predominant one with remarkable increase (4.8 times of control) on day 7. TGF-B1 Mrna showed more striking increase (9.3 times of control) also on day 7. The 2.6-kb transcript of PDGF B and TGF-B1 Mrna increased concurrently with their peak on day 7 in murine anti-Thy-1 nephritis, and the change was more marked in TGF-B1 mRNA. |
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