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| Gradient - gel - SDS - PAGE를 이용한사구체 질환에서의 저분자량의 요단백 분석 |
| 남궁미경 , 이해용 , 임백근 , 김종수 |
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| Abstract |
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Urinary proteins have different patterns according to the deposit site of the CIC rather than to amounts of the urinary protein in immune-complex-glomerular diseases. Gradient-gel-SDS-PAGE is a good method for detecting of the low molecular weight (LMW) urinary pro- teins. We studied the LMW-urinary proteins of common renal diseases in childhood. The results were as follows: LMW urinary proteins were distributed from 66 kD to 29 kD in normal & HSP urine samples. Tubular proteinuria appeared in 1/4 of the cases of AGN, in 3/5 of the cases of IgA glomerulonephropathy and in 2/3 of the cases of minimal change nephrotic syndrome. However, there were no histological differences between tubular proteiriuria and non-tubuloproteinuria of IgA nephropathy. The bands which the researcher observed to be specific were as follows: 17 kD in AGN, 20 kD and ll kD in IgA nephropathy, and 14 kD and 12 kD in minimal change nephrotic syndrome. What the specific bands of LMW urinary protein in common renal diseases by gradient-gel-SDS-PAGE are should be definetly determined through further research. |
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