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| 막성 사구체 신염 환자에서의 자가항원 분리 |
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| Abstract |
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Glomerulonephritis can develop when antibody reacts either with insoluble tissue-fixed glomerular antigens (structural or planted) or when soluble antigens (exogenous or endogenous) and antibodies in the circulation form immune complexes that subsequently accumulate in glomeruli. Membranous glomerulonephritis is known as the typical autoimmune disease in kidney diseases. This experi- ment reports the result of isolation of an autoantigen in the idiopathic membranous glomerulonephritis. The serum from 10 patients of idiopathic membranous glomerulonephritis confirmed by renal biopsy was used as the circulating primary antibody. After electrophor- esis and Western blot with crude extraction of nephrectomized normal human kidney due to traffic accident, the primary antibody (patient's and normal control serum) was added to the tranblotted nitrocellulose membrane which contained the normal human kidney tissue. And then secondary antibody of phosphate labelled affinity purified antobody to human IgG (y) was tagged to the primary antobody on the transblotted membrane and stained by BCIP (5-bromo-4-chloro-3 -indolyl-phosphate)/NBT (nitroblue tetrazolium). The 43 kD band was identified on the immunoblotting of the serum from 7 of 10 idiopathic membranous glomeluronephritis patients, not from normal control serum. |
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