Korean Journal of Nephrology 1994;13(3):454-465.

BCG Vaccine이 ddY마우스의 자연발생 IgA 신증에 미치는 효과

장희경 , 이선경

Abstract

The purpose of this experimental study was to eluci- date the pathogenesis of IgA nephropathy. The animals used in this study were ddY female mice at the age of 9 weeks, as an animal model of spontaneous IgA nephropathy, and were fed rodent laboratory chow and divided into antural course group and BCG vaccinated group. Each of 5 animal was sacrificed at 10, 17, 27, 40 and 49 weeks of age. Just before sacrifices, urine and peripheral blood of the animal were obtained and immediately after sacrifices the spleen and the kidney were removed. And then urinalysis for proteinuria and hematuria, measurement of serum immunoglobulin levels and proportions of T-cell subsets in splenocytes and examination of pathological changes in glomeruli were carried out. The results obtained were summarized as follows: 1) The serum IgA levels of natural course and BCG vaccinated groups at 40 weeks of age were markedly increased than that of natural course group at 10 weeks of age, and serum IgA level of BCG vaccinated group at 40 weeks of age. 2) The mean percentage of CD3+, CD4+, and CDS+ T-cells in spleen of natural course group were not changed significantly with time trends. However, the mean percentage of CD4+ T-cells of BCG vaccinated group was markedly increased. 3) The glomeruli of ddY mice after the age of 40 weeks showed lesions very similar to human IgA nephropathy. These lesions in BCG vaccinated group were more slowly progressed than those of natural course group. 4) In the mesangial area of glomeruli there were deposits of IgA simultaneously evidence for activation of the classic complement pathway. On the basis of the above findings it was concluded that BCG vaccination enhances the count of CD4+ T- cells, and depresses the increasing tendency of serum IgA level and deposition of IgA in mesangial area with time.